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Publication : Interferon Alpha Subtype-Specific Suppression of HIV-1 Infection In Vivo.

First Author  Lavender KJ Year  2016
Journal  J Virol Volume  90
Issue  13 Pages  6001-13
PubMed ID  27099312 Mgi Jnum  J:234495
Mgi Id  MGI:5790131 Doi  10.1128/JVI.00451-16
Citation  Lavender KJ, et al. (2016) Interferon Alpha Subtype-Specific Suppression of HIV-1 Infection In Vivo. J Virol 90(13):6001-13
abstractText  Although all 12 subtypes of human interferon alpha (IFN-alpha) bind the same receptor, recent results have demonstrated that they elicit unique host responses and display distinct efficacies in the control of different viral infections. The IFN-alpha2 subtype is currently in HIV-1 clinical trials, but it has not consistently reduced viral loads in HIV-1 patients and is not the most effective subtype against HIV-1 in vitro We now demonstrate in humanized mice that, when delivered at the same high clinical dose, the human IFN-alpha14 subtype has very potent anti-HIV-1 activity whereas IFN-alpha2 does not. In both postexposure prophylaxis and treatment of acute infections, IFN-alpha14, but not IFN-alpha2, significantly suppressed HIV-1 replication and proviral loads. Furthermore, HIV-1-induced immune hyperactivation, which is a prognosticator of disease progression, was reduced by IFN-alpha14 but not IFN-alpha2. Whereas ineffective IFN-alpha2 therapy was associated with CD8(+) T cell activation, successful IFN-alpha14 therapy was associated with increased intrinsic and innate immunity, including significantly higher induction of tetherin and MX2, increased APOBEC3G signature mutations in HIV-1 proviral DNA, and higher frequencies of TRAIL(+) NK cells. These results identify IFN-alpha14 as a potent new therapeutic that operates via mechanisms distinct from those of antiretroviral drugs. The ability of IFN-alpha14 to reduce both viremia and proviral loads in vivo suggests that it has strong potential as a component of a cure strategy for HIV-1 infections. The broad implication of these results is that the antiviral efficacy of each individual IFN-alpha subtype should be evaluated against the specific virus being treated. IMPORTANCE: The naturally occurring antiviral protein IFN-alpha2 is used to treat hepatitis viruses but has proven rather ineffective against HIV in comparison to triple therapy with the antiretroviral (ARV) drugs. Although ARVs suppress the replication of HIV, they fail to completely clear infections. Since IFN-alpha acts by different mechanism than ARVs and has been shown to reduce HIV proviral loads, clinical trials are under way to test whether IFN-alpha2 combined with ARVs might eradicate HIV-1 infections. IFN-alpha is actually a family of 12 distinct proteins, and each IFN-alpha subtype has different efficacies toward different viruses. Here, we use mice that contain a human immune system, so they can be infected with HIV. With this model, we demonstrate that while IFN-alpha2 is only weakly effective against HIV, IFN-alpha14 is extremely potent. This discovery identifies IFN-alpha14 as a more powerful IFN-alpha subtype for use in combination therapy trials aimed toward an HIV cure.
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