| First Author | Fritzius T | Year | 2006 |
| Journal | Biochem J | Volume | 399 |
| Issue | 1 | Pages | 9-20 |
| PubMed ID | 16792529 | Mgi Jnum | J:225409 |
| Mgi Id | MGI:5693231 | Doi | 10.1042/BJ20060511 |
| Citation | Fritzius T, et al. (2006) A WD-FYVE protein binds to the kinases Akt and PKCzeta/lambda. Biochem J 399(1):9-20 |
| abstractText | WD (tryptophan-aspartic acid dipeptide)-repeat proteins play a central role in signal transduction cascades by co-ordinating the interaction of key signalling molecules. We identified a novel propeller-FYVE [domain identified in Fab1p, YOTB, Vac1p and EEA1 (early endosome antigen 1)] protein, ProF, which is expressed in various cell lines and tissues and consists of seven WD-repeats and a FYVE domain. WD-repeat proteins offer a platform for protein-protein interactions by folding into a seven-bladed propeller-like structure, while the FYVE domain binds to phosphatidylinositol 3-phosphate present mainly on intracellular membranes. The ProF protein partially co-localizes with EEA1 on vesicular structures and binds to the protein kinases Akt and PKCzeta/lambda (protein kinase Czeta/lambda) via its WD-repeat propeller. ProF interacts more strongly with the kinases after hormonal stimulation. Endogenously expressed ProF and the two kinases interact in brain and in the preadipocyte cell line 3T3-L1, suggesting a role in secretory vesicular processes. In summary, we describe a new binding partner for kinases, located on vesicular structures in specialized cells, which may play a role for the spatial organization of signalling cascades. |
