| First Author | Wang Q | Year | 2020 |
| Journal | J Biol Chem | Volume | 295 |
| Issue | 21 | Pages | 7213-7223 |
| PubMed ID | 32229584 | Mgi Jnum | J:298952 |
| Mgi Id | MGI:6450349 | Doi | 10.1074/jbc.RA119.011534 |
| Citation | Wang Q, et al. (2020) The regulatory G protein signaling complex, Gbeta5-R7, promotes glucose- and extracellular signal-stimulated insulin secretion. J Biol Chem 295(21):7213-7223 |
| abstractText | G protein-coupled receptors (GPCRs) are important modulators of glucose-stimulated insulin secretion, essential for maintaining energy homeostasis. Here we investigated the role of Gbeta5-R7, a protein complex consisting of the atypical G protein beta subunit Gbeta5 and a regulator of G protein signaling of the R7 family. Using the mouse insulinoma MIN6 cell line and pancreatic islets, we investigated the effects of G protein subunit beta 5 (Gnb5) knockout on insulin secretion. Consistent with previous work, Gnb5 knockout diminished insulin secretion evoked by the muscarinic cholinergic agonist Oxo-M. We found that the Gnb5 knockout also attenuated the activity of other GPCR agonists, including ADP, arginine vasopressin, glucagon-like peptide 1, and forskolin, and, surprisingly, the response to high glucose. Experiments with MIN6 cells cultured at different densities provided evidence that Gnb5 knockout eliminated the stimulatory effect of cell adhesion on Oxo-M-stimulated glucose-stimulated insulin secretion; this effect likely involved the adhesion GPCR GPR56. Gnb5 knockout did not influence cortical actin depolymerization but affected protein kinase C activity and the 14-3-3 substrate. Importantly, Gnb5 (-/-) islets or MIN6 cells had normal total insulin content and released normal insulin amounts in response to K(+)-evoked membrane depolarization. These results indicate that Gbeta5-R7 plays a role in the insulin secretory pathway downstream of signaling via all GPCRs and glucose. We propose that the Gbeta5-R7 complex regulates a phosphorylation event participating in the vesicular trafficking pathway downstream of G protein signaling and actin depolymerization but upstream of insulin granule release. |
