| First Author | Inoue T | Year | 1990 |
| Journal | J Biol Chem | Volume | 265 |
| Issue | 31 | Pages | 19065-70 |
| PubMed ID | 1699939 | Mgi Jnum | J:10811 |
| Mgi Id | MGI:59256 | Doi | 10.1016/s0021-9258(17)30625-7 |
| Citation | Inoue T, et al. (1990) Isolation of complementary DNAs encoding a cerebellum-enriched nuclear factor I family that activates transcription from the mouse myelin basic protein promoter. J Biol Chem 265(31):19065-70 |
| abstractText | A nuclear factor I (NFI) binding sequence in the myelin basic protein (MBP) promoter is required for brain-specific transcription. In the present study, we isolated multiple cDNAs encoding a family of NFI, NFI-B, from mouse brain cDNA libraries. These clones contain unique nucleotide sequences at their 5', 3', or middle portions, presumably as a result of alternative splicing. The entire sequence of NFI-B suggests that NFI-B is a mouse counterpart of NFI-L, which was previously isolated from rat liver. NFI-B is highly enriched in the cerebellum, in contrast with a ubiquitous NFI, CTF/NF-I. NFI-B proteins translated in vitro could bind to the MBP NFI site. Furthermore, we demonstrated by transfection experiments that NFI-B activated transcription from transfected MBP promoter in neuronal/glial NG108-15 cells. |
