| First Author | Hanada H | Year | 1991 |
| Journal | Biochem Biophys Res Commun | Volume | 176 |
| Issue | 3 | Pages | 1062-7 |
| PubMed ID | 1828149 | Mgi Jnum | J:20078 |
| Mgi Id | MGI:68191 | Doi | 10.1016/0006-291x(91)90391-j |
| Citation | Hanada H, et al. (1991) Molecular cloning of cDNA encoding the 16 KDa subunit of vacuolar H(+)-ATPase from mouse cerebellum. Biochem Biophys Res Commun 176(3):1062-7 |
| abstractText | cDNA for the 16 kDa subunit of vacuolar H(+)-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed. |
