| First Author | Nakamura I | Year | 1997 |
| Journal | FEBS Lett | Volume | 401 |
| Issue | 2-3 | Pages | 207-12 |
| PubMed ID | 9013888 | Mgi Jnum | J:38754 |
| Mgi Id | MGI:86140 | Doi | 10.1016/s0014-5793(96)01454-8 |
| Citation | Nakamura I, et al. (1997) Lack of vacuolar proton ATPase association with the cytoskeleton in osteoclasts of osteosclerotic (oc/oc) mice. FEBS Lett 401(2-3):207-12 |
| abstractText | We examined the pathogenetic mechanism underlying the lack of bone resorption in osteosclerotic oc/oc mice. An immunoelectron microscopic analysis revealed that in the osteoclasts of these mice, no ruffled borders formed, and that vacuolar H+-ATPase (V-ATPase) was present throughout the cytoplasm but not on the apical membranes. The activity of V-ATPase in oc/oc mice was similar to that in normal mice. In normal spleen cell-derived osteoclast-like cells (OCLs), immunoreactivity for V-ATPase was detected in association with Triton X-100-insoluble cellular structure, but not in oc/oc spleen cell-derived OCLs. Moreover, in renal proximal convoluted tubules of oc/oc mice, the basal striation did not form. These results suggest that osteosclerosis in oc/oc mice is possibly due to the dissociation of V-ATPase and cytoskeleton in osteoclasts. |
