| First Author | Lee SW | Year | 1999 |
| Journal | Biochim Biophys Acta | Volume | 1445 |
| Issue | 1 | Pages | 165-71 |
| PubMed ID | 10209270 | Mgi Jnum | J:54630 |
| Mgi Id | MGI:1335628 | Doi | 10.1016/s0167-4781(99)00030-5 |
| Citation | Lee SW, et al. (1999) Cloning of mouse sepiapterin reductase gene and characterization of its promoter region. Biochim Biophys Acta 1445(1):165-71 |
| abstractText | We have isolated and characterized approximately 5 kb mouse sepiapterin reductase gene (Spr) and a highly homologous pseudogene (Sprp). The authentic Spr gene is present as a single copy in the mouse genome and is composed of three exons containing the entire coding region. The primer extension experiment located the transcription initiation site in a putative pyrimidine-rich Inr element. The promoter region of the Spr gene is embedded within a CpG island. It was shown that the promoter region is devoid of distinctive TATA and CAAT boxes. Transient transfection of a series of 5' deletion derivatives of the Spr promoter showed the sequence between -83 and -51 to be essential for promoter activity. The pseudogene Sprp lacks promoter region and exon 3. |
