| First Author | Nagafuchi S | Year | 1999 |
| Journal | FEBS Lett | Volume | 455 |
| Issue | 1-2 | Pages | 101-4 |
| PubMed ID | 10428481 | Mgi Jnum | J:56459 |
| Mgi Id | MGI:1342965 | Doi | 10.1016/s0014-5793(99)00801-7 |
| Citation | Nagafuchi S, et al. (1999) Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission. FEBS Lett 455(1-2):101-4 |
| abstractText | A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(beta)g7 haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. |
