| First Author | Robbins J | Year | 1992 |
| Journal | J Virol | Volume | 66 |
| Issue | 4 | Pages | 2594-9 |
| PubMed ID | 1312643 | Mgi Jnum | J:1812 |
| Mgi Id | MGI:50336 | Doi | 10.1128/jvi.66.4.2594-2599.1992 |
| Citation | Robbins J, et al. (1992) Mouse mammary tumor gene int-3: a member of the notch gene family transforms mammary epithelial cells. J Virol 66(4):2594-9 |
| abstractText | Expression of a 2.3-kb RNA species is induced in mammary tumors as a consequence of insertional mutagenesis at the int-3 locus by the mouse mammary tumor virus. The nucleotide sequence and biological activity of this mammary tumor-specific int-3 RNA species were determined. It contains an open reading frame which encodes a 57-kDa protein. The translated protein possesses six nearly contiguous 32-amino-acid repeats which are related to a similar motif in the Saccharomyces cerevisiae cdc-10-encoded cell cycle protein. In addition, the int-3 cdc-10 repeats are bounded by the PEST amino acid sequence motif which is commonly found in proteins having a rapid turnover and may represent sites for phosphorylation. The int-3 cdc-10 repeat sequences are 50% identical to a portion of the intracellular domain of the neurogenic Drosophila notch gene product. Activation of expression of a recombinant int-3 genomic DNA fragment encoding the 2.3-kb RNA species in HC11 mouse mammary epithelial cells in vitro induces anchorage-independent growth in soft agar. |
