Primary Identifier | MGI:5442346 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase | Gene | Tcf21 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
description | The construct was electroporated into 129SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice to establish a colony.These mice were subsequently bred to Tg(ACTFLPe)9205Dym mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. |
molecularNote | A targeting vector was designed to replace exon 1 of the transcription factor 21,Tcf21 (capsulin; or epicardin) with a mercremer protein followed by a frt-flanked neo cassette. cre recombinase cDNA sequence flanked on each end with a mutated murine estrogen receptor (mer) ligand-binding domain. Flp-mediated recombination deletes the neo cassette. |