| First Author | Li ZD | Year | 1997 |
| Journal | Cell Res | Volume | 7 |
| Issue | 2 | Pages | 161-70 |
| PubMed ID | 9444395 | Mgi Jnum | J:47207 |
| Mgi Id | MGI:1202751 | Doi | 10.1038/cr.1997.17 |
| Citation | Li ZD, et al. (1997) Construction and diversity analysis of a murine IgE phage surface display library. Cell Res 7(2):161-70 |
| abstractText | To make further investigation of the IgE antibody repertoire in Trichosanthin (TCS) allergic responses, a murine IgE phage surface display library was constructed (3.0 x 10(5) independent clones). We first constructed the V epsilon cDNA library (4.6 x 10(5) independent clones) and V kappa cDNA library (3.0 x 10(5) independent clones). Then, the V epsilon and V kappa gene segments were amplified from both libraries by PCR respectively, and assembled into Fab fragment by SOE PCR. The phage library containing Fabs was thus constructed. The diversity of V epsilon from this library was analyzed and proved. Fab clones with high specificity to TCS have been screened out. |
