| First Author | Monach PA | Year | 1995 |
| Journal | Immunity | Volume | 2 |
| Issue | 1 | Pages | 45-59 |
| PubMed ID | 7600302 | Mgi Jnum | J:49488 |
| Mgi Id | MGI:1289113 | Doi | 10.1016/1074-7613(95)90078-0 |
| Citation | Monach PA, et al. (1995) A unique tumor antigen produced by a single amino acid substitution. Immunity 2(1):45-59 |
| abstractText | Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We isolated CD4+ T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein. |
