| First Author | Li L | Year | 2014 |
| Journal | J Neurosci | Volume | 34 |
| Issue | 24 | Pages | 8246-58 |
| PubMed ID | 24920628 | Mgi Jnum | J:211705 |
| Mgi Id | MGI:5576071 | Doi | 10.1523/JNEUROSCI.0278-14.2014 |
| Citation | Li L, et al. (2014) The basal thermal sensitivity of the TRPV1 ion channel is determined by PKCbetaII. J Neurosci 34(24):8246-58 |
| abstractText | Peripheral nociceptors are excited by the activation of membrane receptors and ion channels. The heat-sensitive TRPV1 ion channel responds to various noxious chemical and thermal stimuli, causing pain and itch. Here, we show that TRPV1 is coexpressed with PKCbetaII in a subset of mouse sensory neurons and that, in these neurons, TRPV1 binds directly to PKCbetaII, leading to the activation and translocation of PKCbetaII. Activated PKCbetaII, in turn, significantly increases the responsiveness of TRPV1 by phosphorylating Thr705. The heat sensitivity of TRPV1 is almost eliminated by either knocking down PKCbetaII or mutating Thr705; however, neither of these manipulations affects the potentiation of TRPV1 caused by the activation of PKCepsilon. PKCbetaII thus acts as an auxiliary subunit of TRPV1 by forming a population-dependent TRPV1 ion channel complex controlling the sensitivity of TRPV1 and setting the threshold for pain and itch. |
