| Primary Identifier | MGI:5792687 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ptchd1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector containing a FRT-site flanked NEO selection cassette was used to insert loxP sites flanking exon 2. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 2. In situ hybridization confirmed the absence of full-length mRNA in the brain. |
