| First Author | Sung YH | Year | 2014 |
| Journal | Genome Res | Volume | 24 |
| Issue | 1 | Pages | 125-31 |
| PubMed ID | 24253447 | Mgi Jnum | J:207844 |
| Mgi Id | MGI:5559808 | Doi | 10.1101/gr.163394.113 |
| Citation | Sung YH, et al. (2014) Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases. Genome Res 24(1):125-31 |
| abstractText | RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations in up to 93% of newborn mice with minimal toxicity. RGEN-induced mutations in the mouse Prkdc gene that encodes an enzyme critical for DNA double-strand break repair resulted in immunodeficiency both in F(0) and F(1) mice. We propose that RGEN-mediated mutagenesis in animals will greatly expedite the creation of genetically engineered model organisms, accelerating functional genomic research. |
