| First Author | Xanthopoulos KG | Year | 1989 |
| Journal | Proc Natl Acad Sci U S A | Volume | 86 |
| Issue | 11 | Pages | 4117-21 |
| PubMed ID | 2726767 | Mgi Jnum | J:33101 |
| Mgi Id | MGI:80582 | Doi | 10.1073/pnas.86.11.4117 |
| Citation | Xanthopoulos KG, et al. (1989) Cell-specific transcriptional control of the mouse DNA-binding protein mC/EBP. Proc Natl Acad Sci U S A 86(11):4117-21 |
| abstractText | The mRNA encoding the mouse homolog of C/EBP, a rat DNA-binding protein that participates in activating a number of genes in hepatocytes, is present in liver cells at a far higher concentration than in most other cells, including spleen, kidney, muscle, and the majority of the brain. However, fat cells and intestinal cells contain 25-50% as much mRNA as liver cells. Run-on experiments show that the basis for the restricted cellular distribution of the mouse C/EBP mRNA is transcriptional regulation of the gene. We also show that disruption of cell-cell contacts incident to liver cell dispersion results in a prompt and extensive reduction in mouse C/EBP transcription as we had earlier shown to be the case for a group of 10 genes transcribed in a hepatocyte-specific fashion. In contrast, breaking cell contacts and plating the hepatocytes in culture leads to a prolonged increase in transcription of the Jun-B gene that encodes a widely distributed transcription factor. These results illustrate that the regulation of expression of a mammalian regulatory protein with limited tissue distribution is controlled at the level of transcription and depends on cell contacts. |
