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Publication : Identification and characterization of a novel alternative splice variant of mouse GMx33alpha/GPP34.

First Author  Nagano-Ito M Year  2007
Journal  Gene Volume  400
Issue  1-2 Pages  82-8
PubMed ID  17629635 Mgi Jnum  J:124259
Mgi Id  MGI:3721184 Doi  10.1016/j.gene.2007.06.007
Citation  Nagano-Ito M, et al. (2007) Identification and characterization of a novel alternative splice variant of mouse GMx33alpha/GPP34. Gene 400(1-2):82-8
abstractText  We have cloned and characterized a novel splice variant of mouse GMx33alpha/Golgi-associated protein of 34 kDa (GPP34), hereby designated GMx33alphaV/GPP34V. This splice variant skips the second and third exons, and the resulting frame shift generates a stop codon in the fourth exon. GMx33alphaV/GPP34V is comprised of 81 amino acid residues derived from the N-terminal end of the full length protein and corresponds to approximately one-third of the full length GMx33alpha/GPP34 sequence with a calculated molecular mass of 8900. In contrast to GMx33alpha/GPP34 mRNA which is expressed at similar levels in various tissues, GMx33alphaV/GPP34V mRNA was differentially expressed when examined by RT-PCR. Compared to other tissues, skeletal muscle showed relatively strong expression of GMx33alphaV/GPP34V mRNA. This splice variant cDNA was also detected in a human cell line.
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