Primary Identifier | MGI:3034029 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Nfkbie |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The entire coding region was replaced with a lacZ gene and associated nuclear localization sequence followed by IRES and promoterless neomycin gene. Northern blot analysis established the absence of transcripts while Western blot analysis demonstrated that no protein product was present. Beta-galactosidase expression was detected in the medulla of the lymph node, and a few cells of the thymus and spleen. |