| First Author | Angulo JF | Year | 1991 |
| Journal | Biochimie | Volume | 73 |
| Issue | 2-3 | Pages | 251-6 |
| PubMed ID | 1715759 | Mgi Jnum | J:11398 |
| Mgi Id | MGI:59834 | Doi | 10.1016/0300-9084(91)90210-r |
| Citation | Angulo JF, et al. (1991) Identification of a mouse cDNA fragment whose expressed polypeptide reacts with anti-recA antibodies. Biochimie 73(2-3):251-6 |
| abstractText | We have previously reported the in vivo detection of a mouse nuclear protein that cross-reacts with antibodies raised against E coli recA protein. Here, we characterize monospecific anti-recA antibodies, their use for the immunological screening of a cDNA expression library and the isolation of a mouse cDNA fragment which codes for a polypeptide recognized by anti-recA antibodies. The cDNA fragment is 601 nucleotide long and was called KIN17(601). It contains an open reading frame coding for a 200 amino acid polypeptide. In kin17(200) polypeptide, there are amino acids identical to those that form one of the major antigenic determinants of recA protein. Kin17(200) polypeptide also displays a significant similarity with the helix 1 motif of several homeoproteins. |
