| First Author | Karlsson L | Year | 1994 |
| Journal | Science | Volume | 266 |
| Issue | 5190 | Pages | 1569-73 |
| PubMed ID | 7985028 | Mgi Jnum | J:88528 |
| Mgi Id | MGI:3033762 | Doi | 10.1126/science.7985028 |
| Citation | Karlsson L, et al. (1994) Reconstitution of an operational MHC class II compartment in nonantigen-presenting cells. Science 266(5190):1569-73 |
| abstractText | Professional antigen-presenting cells (APCs) have a distinct compartment in which class II molecules are proposed to acquire antigenic peptides. Genetic evidence suggests that human leukocyte antigen (HLA)-DM, an unusual class II molecule, participates in this process. Peptide acquisition was reconstituted in nonprofessional APCs by transfection of class II, invariant chain (li), and H-2M, the murine equivalent of DM. The H-2M heterodimer appeared in an endosomal compartment, not at the cell surface, and the localization was independent of li. The data presented show that H-2M, class II, and li are the minimally required components for efficient formation of stable class II-peptide complexes, and thus for a functional class II compartment. |
