| First Author | Chiu HS | Year | 2012 |
| Journal | Genesis | Volume | 50 |
| Issue | 9 | Pages | 711-6 |
| PubMed ID | 22511315 | Mgi Jnum | J:187855 |
| Mgi Id | MGI:5438456 | Doi | 10.1002/dvg.22032 |
| Citation | Chiu HS, et al. (2012) Production of a mouse line with a conditional Crim1 mutant allele. Genesis 50(9):711-6 |
| abstractText | Crim1 is a developmentally expressed, transmembrane protein essential for normal embryonic development. We generated mice engineered to contain a Crim1 conditional null allele by flanking exons three and four of Crim1 with unidirectional LoxP sites. After crossing Crim1(+/FLOX) mice with a CMV-Cre line, a Crim1(+/Deltaflox) colony was established after germline transmission of the deleted allele. We then analyzed genomic DNA, mRNA transcripts, and protein expression from Crim1(Deltaflox/Deltaflox) null mice to confirm the nature of the genomic lesion. Crim1(Deltaflox/Deltaflox) mice displayed phenotypes similar to those previously described for a Crim1 gene-trap mutant, Crim1(KST264/KST264) , including perinatal lethality, digit syndactyly, eye, and kidney abnormalities, with varying penetrance and severity. The production of a conditional mutant allele represents a valuable resource for the study of the tissue-specific roles for Crim1, and for understanding the pleimorphic phenotypes associated with Crim1 mutation. genesis 50:711-716, 2012. (c) 2012 Wiley Periodicals, Inc. |
