| First Author | Kretschmer K | Year | 2002 |
| Journal | Eur J Immunol | Volume | 32 |
| Issue | 8 | Pages | 2317-27 |
| PubMed ID | 12209645 | Mgi Jnum | J:135866 |
| Mgi Id | MGI:3794693 | Doi | 10.1002/1521-4141(200208)32:8<2317::AID-IMMU2317>3.0.CO;2-0 |
| Citation | Kretschmer K, et al. (2002) Strong antigenic selection shaping the immunoglobulin heavy chain repertoire of B-1a lymphocytes in lambda 2(315) transgenic mice. Eur J Immunol 32(8):2317-27 |
| abstractText | The B lymphocyte compartment of the recombinant mouse line L2 consists predominantly of CD5(+) B-1 cells that exclusively express the transgenic (Tg) lambda 2 L chain of the plasmacytoma MOPC315. Using such Tg mice as a simplified model to study positive selection processes, we show that restriction to a single L chain results in a strongly oligoclonal IgH chain repertoire in fetal and neonatal liver-derived B cells, as well as in peritoneal CD5(+) B-1 lymphocytes from adult mice. In contrast, in vitro differentiated fetal liver Pro/PreB-I cells from L2 embryos show a clear increase of complementarity determining region three (CDR3) diversity. These data suggest that the antibody repertoire of B-1a cells is strongly selected by antigen during fetal as well as adult peritoneal phase. |
