| First Author | Disney JE | Year | 1989 |
| Journal | J Cell Biol | Volume | 109 |
| Issue | 5 | Pages | 1975-82 |
| PubMed ID | 2808516 | Mgi Jnum | J:10085 |
| Mgi Id | MGI:58542 | Doi | 10.1083/jcb.109.5.1975 |
| Citation | Disney JE, et al. (1989) High-mobility group protein HMG-I localizes to G/Q- and C-bands of human and mouse chromosomes. J Cell Biol 109(5):1975-82 |
| abstractText | Mammalian metaphase chromosomes can be identified by their characteristic banding pattern when stained with Giemsa dye after brief proteolytic digestion. The resulting G-bands are known to contain regions of DNA enriched in A/T residues and to be the principal location for the L1 (or Kpn 1) family of long interspersed repetitive sequences in human chromosomes. Here we report that antibodies raised against a highly purified and biochemically well characterized nonhistone High-Mobility Group protein, HMG-I, specifically localize this protein to the G-bands in mammalian metaphase chromosomes. In some preparations in which chromosomes are highly condensed, HMG-I appears to be located at the centromere and/or telomere regions of mammalian chromosomes as well. To our knowledge, this is the first well-characterized mammalian protein that localizes primarily to G-band regions of chromosomes. |
