| First Author | Bemark M | Year | 1998 |
| Journal | Immunogenetics | Volume | 47 |
| Issue | 3 | Pages | 183-95 |
| PubMed ID | 9435336 | Mgi Jnum | J:45965 |
| Mgi Id | MGI:1196759 | Doi | 10.1007/s002510050347 |
| Citation | Bemark M, et al. (1998) Conserved sequence elements in K promoters from mice and humans: implications for transcriptional regulation and repertoire expression. Immunogenetics 47(3):183-95 |
| abstractText | Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were strictly conserved. Furthermore, there was also sequence conservation immediately 3' of the octamer where either an A or a G residue was conserved. In addition, other DNA elements were also conserved both within the Igk-V subgroups/families and between mouse and human promoters from related subgroups/families. In several of the subgroups/families an E box of the E2A type was conserved 5' of the octamer and a CCCT element was conserved within the IGKV subgroup II and its related mouse Igk-V families. We conclude from this study that conservation of additional sequence elements besides the octamer is a common feature in Igk-V promoters but that distinct elements are conserved only within a given subgroup/family. Thus, the conservation appears to have operated at the level of function rather than at the level of recognition sequence for defined transcription factors. |
