| Primary Identifier | MGI:3820621 | Allele Type | Targeted |
| Gene | Sell | Transmission | Germline |
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | Is Recombinase | false |
| Is Wild Type | false |
| description | The name L(E)SAME describes mice that are compound heterozygote for the Selltm2Tft and Selltm2.1Tft alleles. |
| molecularNote | The membrane-proximal region was replaced with DNA encoding a floxed neomycin resistance gene and the 7 amino acid (APVVPTR) membrane-proximal region of mouse E-selectin. It was predicted that this mutation would prevent cleavage of the protein upon cellular activation. Cell surface levels of mutated protein in homozygote blood, spleen, and lymphocytes was 27%, 34%, and 52%, respectively, of wild-type controls as determined by flow cytometry. Mutated protein was not cleaved from the cell surface upon cellular activation of lymphocytes. |
