| First Author | Kleene KC | Year | 1985 |
| Journal | Biochemistry | Volume | 24 |
| Issue | 3 | Pages | 719-22 |
| PubMed ID | 2986684 | Mgi Jnum | J:7850 |
| Mgi Id | MGI:56319 | Doi | 10.1021/bi00324a027 |
| Citation | Kleene KC, et al. (1985) Nucleotide sequence of a cDNA clone encoding mouse protamine 1. Biochemistry 24(3):719-22 |
| abstractText | The nucleotide sequence of a 404-base cDNA encoding the cysteine-rich, tyrosine-containing mouse protamine has been determined. This insert, isolated from a mouse testis cDNA library, encodes a polypeptide of 50 amino acids of which 28 are arginine, 9 are cysteine, and 3 are tyrosine. The insert contains the complete 3' noncoding region of 151 bases and most of the 5' noncoding region. The predicted amino acid sequence of mouse protamine 1 is about 80% homologous to boar protamine and 67% homologous to bull protamine and contains the central, highly basic domain of four arginine clusters found in the trout protamines. The identification of a cDNA clone for a mouse protamine will facilitate studies of the evolution, regulation, and protein-DNA interaction of this nuclear protein unique to haploid spermatogenic cells. |
