| First Author | Yu J | Year | 1995 |
| Journal | Biochim Biophys Acta | Volume | 1255 |
| Issue | 3 | Pages | 344-50 |
| PubMed ID | 7734452 | Mgi Jnum | J:24525 |
| Mgi Id | MGI:72263 | Doi | 10.1016/0005-2760(95)00015-5 |
| Citation | Yu J, et al. (1995) Cloning and characterization of the mouse PIG-A gene. Biochim Biophys Acta 1255(3):344-50 |
| abstractText | Currently there is no experimental animal model for studying paroxysmal nocturnal hemoglobinuria (PNH), an acquired hemolytic anemia linked to mutations of the PIG-A gene. In this study, we cloned and characterized the mouse PIG-A gene. Sequencing of mouse PIG-A cDNA showed that it encodes a 485 amino acid-long protein. Northern hybridizations identified a major mRNA transcript of 3.6 kb and PCR amplifications identified four smaller alternative splice products. Exon:intron junctional analyses of the mouse PIG-A genome showed 6 exons (1(> or = 60 bp), 2(780 bp), 3(133 bp) 4(133 bp), 5(207 bp), and 6(2276 bp)), the latter 5 of which encompass the coding region. Chromosomal mapping using C57BL/6J x M. Spretus backcross DNA localized the mouse PIG-A gene near the telomeric end of the mouse X chromosome. The isolation of the mouse PIG-A gene opens the possibility for the development of a mouse model of PNH. |
