| Primary Identifier | MGI:3056923 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Slc6a9 |
| Transmission | Germline | Strain of Origin | C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| description | Western blot analysis revealed that whole-brain protein extracts from adult heterozygous mutant mice contain approximately 50% the level of receptor protein in extracts from wild-type mice. While the pattern of immunohistologically-staining receptor distribution throughout the brain is the same in heterozygous and wild-type adult mice, the level of immunoreactive protein is significantly and proportionately reduced in all brain regions of the heterozygotes. |
| molecularNote | Transient Cre expression in embryonic stem cells whose endogenous gene bore previous modifications, including loxP sites flanking a 2000-base pair segment, resulted in deletion of exons 5 through 11, which encode most of the region common to all three receptor isoforms; this mutant allele also contains a previously introduced frt-flanked Pgk-neomycin phosphotransferase cassette in intron 11 and a deletion of 3.9 kb from intron 4. |
