Primary Identifier | MGI:6459287 | Allele Type | Targeted |
Attribute String | Conditional ready, Reporter | Gene | Tlr9 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector contains a loxP-flanked STOP cassette upstream of exon 2, an internal ribosome entry site (IRES) fused to a green fluorescent protein (GFP) sequence inserted downstream of exon 2, and a mutation was introduced in exon 2, in the transmembrane domain. The construct also contains an FRT-flanked neomycin resistance (neo) cassette. Flp-mediated recombination removed the FRT-flanked neo cassette. |