| First Author | Shen Q | Year | 2007 |
| Journal | J Biosci Bioeng | Volume | 104 |
| Issue | 1 | Pages | 14-21 |
| PubMed ID | 17697978 | Mgi Jnum | J:128729 |
| Mgi Id | MGI:3767915 | Doi | 10.1263/jbb.104.14 |
| Citation | Shen Q, et al. (2007) Generation and characterization of islet cell tumor in pTet-on/pTRE-SV40Tag double-transgenic mice model. J Biosci Bioeng 104(1):14-21 |
| abstractText | A line of double-transgenic mice that develop neoplasms arising primarily in the pancreas was established. In these mice, the oncogene SV40 T antigen (Tag) was detected in the pancreas with and without the control of Tet-on system. The transgenic mice that developed pancreatic tumors as early as 20 weeks of age showed hypoglycemia on a blood glucose test. Pathological and immunohistochemical characterizations demonstrated that the tumors belonged to neuroendocrine neoplasms arising from pancreatic islets. A change in IGFs/IGF-1R signaling pathway was detected using real-time PCR analysis. A potential association between the IGFs/IGF-1R system and SV40Tag was studied to further explain the cancerogenesis of the double-transgenic mice by Western blot analysis and immunoprecipitation experiments. The results suggest that a Tag transgenic mice model could be used to study the molecular mechanism of the tumorigenesis of islets. |
