| First Author | Cao C | Year | 2015 |
| Journal | J Biol Chem | Volume | 290 |
| Issue | 35 | Pages | 21642-51 |
| PubMed ID | 26160172 | Mgi Jnum | J:225500 |
| Mgi Id | MGI:5693443 | Doi | 10.1074/jbc.M115.645796 |
| Citation | Cao C, et al. (2015) Mac-1 Regulates IL-13 Activity in Macrophages by Directly Interacting with IL-13Ralpha1. J Biol Chem 290(35):21642-51 |
| abstractText | Mac-1 exhibits a unique inhibitory activity toward IL-13-induced JAK/STAT activation and thereby regulates macrophage to foam cell transformation. However, the underlying molecular mechanism is unknown. In this study, we report the identification of IL-13Ralpha1, a component of the IL-13 receptor (IL-13R), as a novel ligand of integrin Mac-1, using a co-evolution-based algorithm. Biochemical analyses demonstrated that recombinant IL-13Ralpha1 binds Mac-1 in a purified system and supports Mac-1-mediated cell adhesion. Co-immunoprecipitation experiments revealed that endogenous Mac-1 forms a complex with IL-13Ralpha1 in solution, and confocal fluorescence microscopy demonstrated that these two receptors co-localize with each other on the surface of macrophages. Moreover, we found that genetic inactivation of Mac-1 promotes IL-13-induced JAK/STAT activation in macrophages, resulting in enhanced polarization along the alternative activation pathway. Importantly, we observed that Mac-1(-/-) macrophages exhibit increased expression of foam cell differentiation markers including 15-lipoxygenase and lectin-type oxidized LDL receptor-1 both in vitro and in vivo. Indeed, we found that Mac-1(-/-)LDLR(-/-) mice develop significantly more foam cells than control LDLR(-/-) mice, using an in vivo model of foam cell formation. Together, our data establish for the first time a molecular mechanism by which Mac-1 regulates the signaling activity of IL-13 in macrophages. This newly identified IL-13Ralpha1/Mac-1-dependent pathway may offer novel targets for therapeutic intervention in the future. |
