| Primary Identifier | MGI:6877071 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout, Reporter | Gene | Ostn |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Targeting vector PG00061_Z_2_D09 from KOMP project was electroporated into ES cells. The targeting cassette is composed of En2 intron/splice-acceptor/exon, an IRES, the beta-galactosidase (LacZ) gene, a polyadenylation signal (pA) a loxP site, a beta-actin promoter, the neomycin-resistance gene (neo), a second pA site, second FRT site, and second loxP site. A third loxP site was inserted downstream of exon 3. In situ hybridization confirmed absence of mRNA in homozygotes. |
