| First Author | Dangond F | Year | 1999 |
| Journal | Mol Cell Biol Res Commun | Volume | 2 |
| Issue | 2 | Pages | 91-6 |
| PubMed ID | 10542131 | Mgi Jnum | J:58415 |
| Mgi Id | MGI:1347643 | Doi | 10.1006/mcbr.1999.0156 |
| Citation | Dangond F, et al. (1999) Cloning and expression of a murine histone deacetylase 3 (mHdac3) cDNA and mapping to a region of conserved synteny between murine chromosome 18 and human chromosome 5. Mol Cell Biol Res Commun 2(2):91-6 |
| abstractText | Histone deacetylases (HDACs) are enzymes that play a pivotal role in transcription, differentiation, and cell cycle progression. We previously cloned human HDAC3 cDNA and showed that its transfection into THP-1 cells results in G2/M cell cycle accumulation. Using bioinformatic screening and PCR, we have now cloned the murine Hdac3 cDNA, which encodes a 428-amino-acid protein with near complete identity to its human ortholog. To establish a link to a potential disease locus, we performed PCR-based chromosomal mapping for the mHdac3 gene and chromosomal fluorescence in situ hybridization (FISH) for the human gene. mHdac3 localizes to chromosome 18 and human HDAC3 gene localizes to a syntenic region in chromosome 5 at band q31.3-q32 telomeric to the cytokine gene cluster. Transfection of mHdac3 into HeLa cells led to accumulation in G2/M. Our results suggest a cell cycle function for murine Hdac3, reflecting the complex regulatory roles of this gene family. |
