| First Author | Kawai S | Year | 1994 |
| Journal | J Biochem | Volume | 115 |
| Issue | 4 | Pages | 641-3 |
| PubMed ID | 8089076 | Mgi Jnum | J:18798 |
| Mgi Id | MGI:66590 | Doi | 10.1093/oxfordjournals.jbchem.a124388 |
| Citation | Kawai S, et al. (1994) Cloning and characterization of OSF-3, a new member of the MER5 family, expressed in mouse osteoblastic cells. J Biochem 115(4):641-3 |
| abstractText | A cDNA library prepared from the mouse osteoblastic cell line, MC3T3-E1, was screened for the presence of specifically expressed genes by employing subtraction hybridization/differential screening methods. A cDNA clone was identified and sequenced, encoding a protein designated as osteoblast specific factor 3 (OSF-3) comprising 199 amino acids. RNA dot blot analysis indicated weak OSF-3 expression in thymus, spleen, brain, lung, testis, and heart, and high expression in kidney and liver. A homology search of an amino acid sequence database revealed a strong relationship of OSF-3 to the MER5 (gene preferentially expression in murine erythroleukemia cells) protein and human pag (proliferation associated gene) product. This indicates that OSF-3 plays an intrinsic role in the proliferation and/or differentiation of bone cells. |
