| First Author | Hirose T | Year | 2015 |
| Journal | J Vet Med Sci | Volume | 77 |
| Issue | 11 | Pages | 1385-9 |
| PubMed ID | 26050750 | Mgi Jnum | J:274240 |
| Mgi Id | MGI:6296944 | Doi | 10.1292/jvms.15-0015 |
| Citation | Hirose T, et al. (2015) Comparative study of dermal components and plasma TGF-beta1 levels in Slc39a13/Zip13-KO mice. J Vet Med Sci 77(11):1385-9 |
| abstractText | Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are identified in the extracellular matrix. Transforming growth factor-beta1 (TGF-beta1) plays a crucial role in formation of the extracellular matrix. It has been reported that the loss of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-beta1 signaling pathway is observed, although the relationship between the dermis abnormalities and peripheral TGF-beta1 level has been unclear. We investigated the characteristics of the dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS. Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the amount of collagen were decreased, and there were very few collagen fibrils with diameters of more than 150 nm in Zip13-KO mice dermis. We also found that the TGF-beta1 level was significantly higher in Zip13-KO mice serum. These results suggest that collagen synthesis and collagen fibril fusion might be impaired in Zip13-KO mice and that the possible decrease of decorin level by reduction of the DS ratio probably caused an increase of free TGF-beta1 in Zip13-KO mice. In conclusion, skin fragility due to defective ZIP13 protein may be attributable to impaired extracellular matrix synthesis accompanied by abnormal peripheral TGF-beta homeostasis. |
