| First Author | Liu J | Year | 2007 |
| Journal | J Biol Chem | Volume | 282 |
| Issue | 26 | Pages | 18945-52 |
| PubMed ID | 17478420 | Mgi Jnum | J:123481 |
| Mgi Id | MGI:3718725 | Doi | 10.1074/jbc.M701083200 |
| Citation | Liu J, et al. (2007) CatSperbeta, a novel transmembrane protein in the CatSper channel complex. J Biol Chem 282(26):18945-52 |
| abstractText | Four CatSper ion channel subunit genes (CatSpers 1-4) are required for sperm cell hyperactivation and male fertility. The four proteins assemble (presumably as a tetramer) to form a sperm-specific, alkalinization-activated Ca(2+)-selective channel. We set out to identify proteins associating with CatSper that might help explain its unique role in spermatozoa. Using a transgenic approach, a CatSper1 complex was purified from mouse testis that contained heat shock protein 70-2, a testis-specific chaperone, and CatSperbeta, a novel protein with two putative transmembrane-spanning domains. Like the CatSper ion channel subunits, CatSperbeta was restricted to testis and localized to the principal piece of the sperm tail. CatSperbeta protein is absent in CatSper1(-/-) sperm, suggesting that it is required for trafficking or formation of a stable channel complex. CatSperbeta is the first identified auxiliary protein to the CatSper channel. |
