| Primary Identifier | MGI:6514365 | Allele Type | Endonuclease-mediated |
| Attribute String | Recombinase | Gene | Sncg |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | CRISPR/cas9 genome editing is used to insert an internal ribosome entry site 2 sequence, a FlpO recombinase gene, a bovine growth hormone polyA sequence, an attB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an attP site following the STOP codon. The guide vector contained the Sncg guide RNA sequences designed for the endogenous stop codon and also a DNA sequence encoding a human codon-optimized Streptococcus pyogenes CRISPR associated protein 9 (SpCas9). Of note, the guide vector is designed so the SpCas9 protein is only expressed in embryonic stem (ES) cells and the SpCas9 DNA sequence does not integrate into the genome |
