| First Author | Kim JH | Year | 2014 |
| Journal | Immunology | Volume | 142 |
| Issue | 3 | Pages | 506-16 |
| PubMed ID | 24628083 | Mgi Jnum | J:212000 |
| Mgi Id | MGI:5577194 | Doi | 10.1111/imm.12282 |
| Citation | Kim JH, et al. (2014) Lack of transglutaminase 2 diminished T-cell responses in mice. Immunology 142(3):506-16 |
| abstractText | Transglutaminase 2 (TG2) has been reported to play a role in dendritic cell activation and B-cell differentiation after immunization. Its presence and role in T cells, however, has not been explored. In the present study, we determined the expression of TG2 on mouse T cells, and evaluated its role by comparing the behaviours of wild-type and TG2(-/-) T cells after activation. In our results, naive T cells minimally expressed TG2, expression of which was increased after activation. T-cell proliferation, expression of activation markers such as CD69 and CD25, and secretions of interleukin-2 and interferon-gamma were suppressed in the absence of TG2, presumably due, in part, to diminished nuclear factor-kappaB activation. These effects on T cells seemed to be reflected in the in vivo immune response, the contact hypersensitivity reaction elicited by 2,4-dinitro-1-fluorobenzene, with lowered peak responses in the TG2(-/-) mice. When splenic T cells from mice immunized with tumour lysate-loaded wild-type dendritic cells were re-challenged ex vivo with the same antigen, the profile of surface markers including CD44, CD62L, and CD127 strongly indicated lesser generation of memory CD8(+) T cells in TG2(-/-) mice. In the TG2(-/-) CD8(+) T cells, moreover, Eomes expression was markedly decreased. These results indicate possible roles of TG2 in CD8(+) T-cell activation and CD8(+) memory T-cell generation. |
