| Primary Identifier | MGI:6196334 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Gsdmd |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Cas9 mRNA and gRNA (5'-AGCATCCTGGCATTCCGAG-3') were co-injected into C57BL/6NCrl zygotes. Founders with frameshift mutations were screened via T7E1 assay and validated by DNA sequencing. This mutation carries an 37 bp deletion in exon 5 of the gene. |
