| First Author | Chakrabarti R | Year | 2018 |
| Journal | EMBO Rep | Volume | 19 |
| Issue | 11 | PubMed ID | 30201800 |
| Mgi Jnum | J:267470 | Mgi Id | MGI:6256687 |
| Doi | 10.15252/embr.201744937 | Citation | Chakrabarti R, et al. (2018) Vesicle sub-pool organization at inner hair cell ribbon synapses. EMBO Rep 19(11) |
| abstractText | The afferent inner hair cell synapse harbors the synaptic ribbon, which ensures a constant vesicle supply. Synaptic vesicles (SVs) are arranged in morphologically discernable pools, linked via filaments to the ribbon or the presynaptic membrane. We propose that filaments play a major role in SV resupply and exocytosis at the ribbon. Using advanced electron microscopy, we demonstrate that SVs are organized in sub-pools defined by the filament number per vesicle and its connections. Upon stimulation, SVs increasingly linked to other vesicles and to the ribbon, whereas single-tethered SVs dominated at the membrane. Mutant mice for the hair cell protein otoferlin (pachanga, Otof (Pga/Pga) ) are profoundly deaf with reduced sustained release, serving as a model to investigate the SV replenishment at IHCs. Upon stimulation, multiple-tethered and docked vesicles (rarely observed in wild-type) accumulated at Otof (Pga/Pga) active zones due to an impairment downstream of docking. Conclusively, vesicles are organized in sub-pools at ribbon-type active zones by filaments to support vesicle supply, transport, and finally release. |
