| First Author | Kim SH | Year | 2021 |
| Journal | Cell Rep | Volume | 35 |
| Issue | 2 | Pages | 108995 |
| PubMed ID | 33852847 | Mgi Jnum | J:313891 |
| Mgi Id | MGI:6716751 | Doi | 10.1016/j.celrep.2021.108995 |
| Citation | Kim SH, et al. (2021) Complement C5a promotes antigen cross-presentation by Peyer's patch monocyte-derived dendritic cells and drives a protective CD8(+) T cell response. Cell Rep 35(2):108995 |
| abstractText | The complement fragment C5a is closely associated with adaptive immune induction in the mucosa. However, the mechanisms that control CD8(+) T cell responses by C5a have not been extensively explored. This study reveals that C5/C5a in the Peyer's patch (PP) subepithelial dome increases upon oral Listeria infection. We hypothesize that C5aR(+) PP cells play an important role in the induction of antigen-specific T cell immunity. Using single-cell RNA sequencing, we identify C5aR- and lysozyme-expressing dendritic cells (C5aR(+) LysoDCs) in PP and examine their role in CD8(+) T cell immune induction. Stimulation of C5aR(+) LysoDCs by C5a increases reactive oxygen species levels, leading to efficient antigen cross-presentation, which elicits an antigen-specific CD8(+) T cell response. In C5-deficient mice, oral co-administration of C5a and Listeria enhances Listeria-specific cytotoxic T cell levels. Collectively, these findings suggest a role of the complement system in intestinal T cell immunity. |
