| First Author | Yoshikawa S | Year | 2019 |
| Journal | Sci Signal | Volume | 12 |
| Issue | 576 | PubMed ID | 30967512 |
| Mgi Jnum | J:284409 | Mgi Id | MGI:6381094 |
| Doi | 10.1126/scisignal.aav2060 | Citation | Yoshikawa S, et al. (2019) Pivotal role of STIM2, but not STIM1, in IL-4 production by IL-3-stimulated murine basophils. Sci Signal 12(576) |
| abstractText | Basophils have nonredundant roles in various immune responses that require Ca(2+) influx. Here, we examined the role of two Ca(2+) sensors, stromal interaction molecule 1 and 2 (STIM1 and STIM2), in basophil activation. We found that loss of STIM1, but not STIM2, impaired basophil IL-4 production after stimulation with immunoglobulin E (IgE)-containing immune complexes. In contrast, when basophils were stimulated with IL-3, loss of STIM2, but not STIM1, reduced basophil IL-4 production. This difference in STIM proteins was associated with distinct time courses of Ca(2+) influx and transcription of the Il4 gene that were elicited by each stimulus. Similarly, basophil-specific STIM1 expression was required for IgE-driven chronic allergic inflammation in vivo, whereas STIM2 was required for IL-4 production after combined IL-3 and IL-33 treatment in mice. These data indicate that STIM1 and STIM2 have differential roles in the production of IL-4, which are stimulus dependent. Furthermore, these results illustrate the vital role of STIM2 in basophils, which is often considered to be less important than STIM1. |
