| First Author | Nakamura T | Year | 1995 |
| Journal | Genomics | Volume | 30 |
| Issue | 2 | Pages | 312-9 |
| PubMed ID | 8586432 | Mgi Jnum | J:29919 |
| Mgi Id | MGI:77444 | Doi | 10.1006/geno.1995.9872 |
| Citation | Nakamura T, et al. (1995) The murine lymphotoxin-beta receptor cDNA: isolation by the signal sequence trap and chromosomal mapping. Genomics 30(2):312-9 |
| abstractText | To isolate novel molecules involved in intercellular signaling during mouse embryogenesis, we employed the signal sequence trap (SST) method, a newly developed strategy for cloning secreted proteins and type I membrane proteins. We constructed an SST cDNA library of mouse embryonic heart mRNA, screened 2000 clones, and acquired 1 positive clone that appeared to contain the signal sequence. Homology searches revealed that this clone encodes the mouse lymphotoxin-beta receptor (LT beta-R). The deduced amino acid sequence of the mouse LT beta-R was 66% identical to that of the human LT beta-R. Northern analysis of various organs in adult mice showed that expression levels of LT beta-R mRNA were strong in lung, liver, and kidney, moderate in heart and testis, but weak in brain, thymus, spleen, and lymph nodes. Since the mouse LT beta-R was already expressed in 7-day-postcoitus embryo, the LT beta/LT beta-R system might have some functions in early embryogenesis. We performed chromosomal mapping of the murine LT beta-R gene by linkage analysis with recombinant inbred mouse strains and found that its locus is very close to the tumor necrosis factor receptor 1 gene on chromosome 6. |
