| First Author | Becker D | Year | 1996 |
| Journal | Pharm Acta Helv | Volume | 71 |
| Issue | 1 | Pages | 29-35 |
| PubMed ID | 8786996 | Mgi Jnum | J:35721 |
| Mgi Id | MGI:83170 | Doi | 10.1016/0031-6865(95)00049-6 |
| Citation | Becker D, et al. (1996) The limb/LPM enhancer of the murine Hoxb6 gene: reporter gene analysis in transgenic embryos and studies of DNA-protein interactions. Pharm Acta Helv 71(1):29-35 |
| abstractText | Homeobox genes are expressed in stage-, region-, and tissue-specific patterns during embryonic development of the mouse. In order to study cis-acting regulatory elements of murine homeobox genes, transgenic mouse embryos were generated which contained the LacZ reporter gene under the control of different fragments from the murine Hoxb6 gene. A 2 kb limb/LPM (lateral plate mesoderm) enhancer region was identified which reproduced several aspects of the endogenous Hoxb6 gene expression pattern in the mesenchyme of the developing limb buds and the ventro-lateral body region. Both tissues represent derivatives of the lateral plate mesoderm. In order to describe proteins interacting with specific DNA sequences in the limb/LPM enhancer, electro mobility shift assays were performed using nuclear extracts prepared from embryonic tissues. Several specific DNA-protein complexes could be detected, suggesting that these DNA-protein interactions are important for the regulation of Hoxb6 gene expression. |
