| Primary Identifier | MGI:3046857 | Allele Type | Targeted |
| Gene | Igh | Transmission | Germline |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The DQ52 element of Igh-D was disrupted by the insertion of a floxed neo cassette and the Igh-J elements were replaced with sequence encoding the variable region of DNA binding antibody D42. In constrast to Ightm3Cgn, this allele contains Igh-V11 mutations resulting in a Ser to Asn substitution at position 35 in CDR1 and an Asn to Tyr substitution at position 53 in CDR2. The Igh intron enhancer was left intact. Flow cytometry verified cell surface expression of the mutant protein product. The floxed neo cassette was removed via cre-mediated recombination. |
