| Primary Identifier | MGI:5496656 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Phf2 |
| Transmission | Germline | Strain of Origin | (129 x C57BL/6)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The construct targeted upstream of exon 6 contains (5' to 3') a loxP site, lacZ gene, FRT site, neomycin resistance cassette, loxP site and FRT site. An additional FRT site was inserted downstream of exon 6. Flp-mediated recombination removed the neomycin resistance cassette and exon 6 but left the lacZ gene intact. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. |
