| First Author | Picard D | Year | 1983 |
| Journal | Proc Natl Acad Sci U S A | Volume | 80 |
| Issue | 2 | Pages | 417-21 |
| PubMed ID | 6300842 | Mgi Jnum | J:20096 |
| Mgi Id | MGI:68209 | Doi | 10.1073/pnas.80.2.417 |
| Citation | Picard D, et al. (1983) Correct transcription of a cloned mouse immunoglobulin gene in vivo. Proc Natl Acad Sci U S A 80(2):417-21 |
| abstractText | We have obtained correct transcripts from the mouse immunoglobulin lambda I light chain gene on transfection into human HeLa cells. Linkage to simian virus 40 (SV40) DNA containing a transcriptional enhancer element was required to raise lambda-chain gene transcription to a detectable level in our transient-expression assay. The transcripts had the same 5' end as authentic lambda I mRNA when the SV40 enhancer element was 150 base pairs upstream from the cap site. The situation was different when the lambda-chain gene promoter was separated from the SV40 sequences by more than 1 kilobase pair of spacer DNA; then, lambda-chain gene transcripts were not correctly initiated in human HeLa, monkey CV-1, and mouse 3T6 cells. In this respect, the lambda-chain gene behaves differently from the rabbit beta-globin gene, which can be activated by the SV40 enhancer over distances of several kilobases. |
