| Primary Identifier | MGI:6201711 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), an FRT-flanked STOP cassette (with a short open reading frame, followed by translational stops in all three reading frames, a synthetic polyA sequence, a HGH polyA sequence and a HSV-TK polyA sequence), a loxP-flanked STOP cassette (with stop codons in all three reading frames linked to three repeats of the SV40 polyA signal), the SypYFP fusion gene (a mouse synaptophysin [Syp] cDNA fused to an enhanced yellow fluorescent protein sequence), a woodchuck hepatitis virus post-transcriptional regulatory element, a BGH polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK pA cassette and an AttP site. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. |
