| First Author | Shotorbani SS | Year | 2012 |
| Journal | Cell Immunol | Volume | 275 |
| Issue | 1-2 | Pages | 61-8 |
| PubMed ID | 22483852 | Mgi Jnum | J:188177 |
| Mgi Id | MGI:5439664 | Doi | 10.1016/j.cellimm.2012.02.016 |
| Citation | Shotorbani SS, et al. (2012) Over-expression of Hlx homeobox gene in DC2.4 dendritic cell enhances its maturation and antigen presentation. Cell Immunol 275(1-2):61-8 |
| abstractText | Hlx as a Th1-specific transcription factor, it appears to drive maturation of Th1 and IFN-gamma secretion in cooperation with T-bet. In this study, we established a stable Hlx-over-expressed dendritic cell line (DC2.4/Hlx), and investigated the possible effect of Hlx gene on maturation of dendritic cell-line (DC2.4). Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-gamma, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II. Functional assay for DC2.4/Hlx showed that over-expressed Hlx increased the expression level of interleukin-12 in the supernatant and decreased DC endocytosis when cells were incubated in vitro. Furthermore, using a syngeneic T cell activation model, we found that DC2.4/Hlx could obviously present ovalbumin (OVA) antigen to T cell in OVA pre-immunized mice. |
