| Primary Identifier | MGI:2684189 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph | Gene | Med1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neomycin resistance gene was placed in intron 2 while an additional loxP site was inserted in exon 1. The resulting construct was a hypomorph expressing 80% less mRNA than normal and with a comparable reduction in protein product. Transient transfection of properly targeted ES cells with a cre expression vector to remove neomycin but leaving exon 1 and 2 in place but flanked by loxP sites also resulted in a hypomorph with an identical phenotypic effect. |
